@Article{HollandaLoLaBeCoZa:2014:GrCaNa,
author = "Hollanda, L. M. and Lobo, A. O. and Lancellotti, M. and Berni, E.
and Corat, Evaldo Jos{\'e} and Zanin, Hudson",
affiliation = "UNICAMP and UNIVAP and UNICAMP and UNICAMP and {Instituto Nacional
de Pesquisas Espaciais (INPE)} and {Instituto Nacional de
Pesquisas Espaciais (INPE)}",
title = "Graphene and carbon nanotube nanocomposite for gene transfection",
journal = "Materials Science and Engineering C",
year = "2014",
volume = "39",
number = "1",
pages = "288--298",
month = "June",
keywords = "BET adsorption isotherms, carbon nanotube nanocomposites, cell
viability, Fourier transform infra reds, graphene oxides, green
fluorescent protein, high-resolution scanning electron
microscopies, transfection efficiency, carbon nanotubes, cell
culture, Cells, Composite materials, DNA, Fluorescence, Gene
transfer, Genes, Graphene, Nanocomposites, Particle size analysis,
Plasma etching, Plasmas, Scanning electron microscopy, Zeta
potential, Molecular biology.",
abstract = "Graphene and carbon nanotube nanocomposite (GCN) was synthesised
and applied in gene transfection of pIRES plasmid conjugated with
green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines.
The tips of the multi-walled carbon nanotubes (MWCNTs) were
exfoliated by oxygen plasma etching, which is also known to attach
oxygen content groups on the MWCNT surfaces, changing their
hydrophobicity. The nanocomposite was characterised by high
resolution scanning electron microscopy; energy-dispersive X-ray,
Fourier transform infrared and Raman spectroscopies, as well as
zeta potential and particle size analyses using dynamic light
scattering. BET adsorption isotherms showed the GCN to have an
effective surface area of 38.5 m2/g. The GCN and pIRES plasmid
conjugated with the GFP gene, forming {\`A}-stacking when
dispersed in water by magnetic stirring, resulting in a helical
wrap. The measured zeta potential confirmed that the plasmid was
connected to the nanocomposite. The NIH-3T3 and NG97 cell lines
could phagocytize this wrap. The gene transfection was
characterised by fluorescent protein produced in the cells and
pictured by fluorescent microscopy. Before application, we studied
GCN cell viability in NIH-3T3 and NG97 line cells using both MTT
and Neutral Red uptake assays. Our results suggest that GCN has
moderate stability behaviour as colloid solution and has great
potential as a gene carrier agent in non-viral based therapy, with
low cytotoxicity and good transfection efficiency.",
doi = "10.1016/j.msec.2014.03.002",
url = "http://dx.doi.org/10.1016/j.msec.2014.03.002",
issn = "0928-4931",
label = "scopus 2014-05 HollandaLoLaBeCoZa:2014:GrCaNa",
language = "en",
targetfile = "1-s2.0-S0928493114001180-main.pdf",
url = "http://dx.doi.org/10.1016/j.msec.2014.03.002",
urlaccessdate = "27 abr. 2024"
}